Project Teams

Project Management: Overall direction and project management of the SSGCID project is overseen by a Scientific Leadership Team comprised of the PIs from each of the five participating sites.  Day-to-day direction of the various teams is coordinated by the SSGCID Project Manager (located at Seattle BioMed) and Site/Project Managers at the other sites. 

Seattle BioMed
Peter Myler (SSGCID Principal Investigator)
Robin Stacy (Senior Project Manager)
Christina McCormick (Administrative Coordinator)

Emerald BioStructures
Lance Stewart (Co-Principal Investigator)
Alex Burgin (Site Manager)
Bart Staker (Project Manager)

UW PPG
Wesley Van Voorhis (Co-Principal Investigator)
Alberto Napuli (Site Manager)
Lynn Barrett (Lab Manager)

UW-NMR
Gabriele Varani (Co-Principal Investigator)

Pacific Northwest National Laboratory
Garry Buchko (Co-Principal Investigator)

IT & Data Management: This team is tasked with ensuring efficient and effective transfer of data and information between all SSGCID members and are primarily involved in design and maintenance of the SSGCID SharePoint site, SSGCID website and the SSGCID Central Target Tracking Database (CTTDB).  Team members are also involved with maintaining site-specific databases.

SeattleBiomed
Peter Myler (SSGCID Principal Investigator)
Tom Blackwell (IT Director)
Isabelle Phan (Senior Bioinformatics Research Specialist)
Lisabeth Cron (Bioinformatics Programmer)
Sandhya Subramanian (Bioinformatics Applications Specialist)
Robin Stacy (Senior Project Manager)

Target Selection: The Target Selection team will carry out two or three rounds of target selection each year, usually resulting in batches of 100-300 protein targets. 

SeattleBiomed
Peter Myler (SSGCID Principal Investigator)
Isabelle Phan (Senior Bioinformatics Research Specialist)
Sandhya Subramanian (Bioinformatics Applications Specialist)

Cloning & Expression Testing: The cloning & expression teams at each site will design PCR primers to amplify or synthesize target genes, clone them into the appropriate vectors, and test them for soluble expression in bacterial, wheat-germ, cell-free, baculovirus and mammalian expression systems.

SeattleBiomed - Cell-free and mammalian expression systems
Elizabeth Mundt

Emerald - Bacterial, baculovirus and mammalian expression systems
Don Lorimer (Director Molecular Biology)
Amy Raymond (Senior Scientist)

UW-PPG - Bacterial and wheat-germ expression systems
Steve Nakazawa Hewitt (Group Manager)
Janhavi Bhandari (Res. Assoc. Scientist)

Protein Production: The protein production teams at each site will grow large-scale preps of solubly-expressed proteins and purify milligram quantities for crystallization screening and NMR-based HSQC analysis. 

SeattleBiomed
Sunny Zhang (Protein Production Core Manager)
WenJin Guo
Kaitlin Thompkins
Elizabeth Mundt
Micah Ferrel
Ariel Abramov
David Sigmon

Emerald
Roger Zabinski (Research Scientist)

UW PPG
Cassie Bryan
Angela Kelley (Research Scientist I)
David Leibley

Crystallization: Crystallization testing of purified proteins from all Protein Production Teams will be carried out at Emerald using a high-throughput robotic system. 

Emerald
Jeff Christiansen (Senior Research Associate)
Tom Edwards (Senior Research Scientist)
Doug Davies (Senior Research Scientist)

Data collection & Structure Solution: This team involves personnel at three sites and includes two different processes (X-ray crystallography and NMR).

Emerald - X-ray crystallography
Bart Staker (Site Manager)
Jan Abendroth (Sr Research Scientist)

UW NMR Group - NMR
Suxin Zheng (Post-doctoral Scientist)
Ravi Barnwal (Research Scientist)

Pacific Northwest National Laboratory - NMR
Garry Buchko (Co-Principal Investigator)