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E-mail submission of Target Requests

SSGCID strongly encourages submission of Target Requests by webform to speed up processing time. However, should the requestor have a large number of targets to submit, we will accept submissions by email.

Applicants should e-mail the following information to target.request@seattlechildrens.org.  Failure to include all of the requested information may results in a delay in consideration of the target request. Please note that our pipeline is most successful with soluble proteins or domains (no trans-membrane regions) shorter than 700 residues and containing at most 10 cysteines.

• Requestor information: the applicant’s name, institution and contact information. 

• Target description: Please provide Uniprot ID(s) for the target(s). If no UniProt ID is available, please provide a hyperlink to the webpage describing the sequence. If there is no webpage available, please include DNA sequence and annotation.

• Area of biomedical interest: Please select the most relevant.

  1. Known drug target – The gene product is the target of a drug which is currently on the market or in development. Minimally, inhibitors of the target will have been shown to block cell growth in the genus/species listed.
  2. Potential drug target – An ortholog of a known drug target, an essential gene or a homolog of an essential gene.
  3. Targets associated with virulence and/or pathogenesis- A gene product associated with pathogen adhesion, colonization, invasion, immune response inhibition or toxicity of the host cells.
  4. Vaccine candidate – A gene product identified as a vaccine candidate.
  5. Targets involved in drug resistance – The gene product is confirmed or suggested to be involved (directly or indirectly) in drug-target binding, drug degradation or drug removal all of which result in evolution over time of drug resistance.
  6. Targets showing phylogenetic evidence for essentiality, virulence or pathogenesis – Targets for which orthologues present in a pathogenic species/strain and absent in a non-pathogenic species/strain.
  7. Targets associated with innate immunity – Human proteins associated with non-adaptive immune mechanisms that recognize, and respond to, microorganisms, microbial products and antigens.
  8. Marker of infection – A biomarker, antigen or other protein used to detect pathogen infection.
  9. Targets of other biological interest – targets falling outside the categories described above.

• Justification: Include a brief (one or two sentence) description of how determination of the target structure(s) will enhance research progress in the field.

• Justification evidence: Describe supporting experimental evidence and/or relevant literature for functional or biological relevance of the target(s) (optional).

• Material Availability:
  - If constructs are available, please provide construct name, sequence and expression information. Does the construct produce soluble protein, if so how much have you documented and under which conditions. List any important post-translational protein modifications, additives or ligands which may improve results.
  - If protein is available, can you provide a minimum of 150 μl at ~5 mg/ml soluble protein to fast-track the request? If so, please provide the expressed protein sequence, vector and tag and list any additional information such as ligands or co-factors bound.

• Intellectual property agreement: Please note that any structures successfully determined by the SSGCID will be submitted to the Protein Data Bank and be part of the public domain.