Overall direction and project management of the SSGCID project is overseen by a Scientific Leadership Team comprised of the PIs from each of the five participating sites. Day-to-day direction of the various teams is coordinated by the SSGCID Project Manager (located at Seattle BioMed) and Site/Project Managers at the other sites.
Center for Infectious Disease Research (CIDResearch)
Peter Myler (Principal Investigator)
Robin Stacy (Senior Project Manager)
Bart Staker (Co-Investigator and Outreach Manager)
Thomas Edwards (Co-Principal Investigator)
Peter Horanyi (Co-Investigator)
Don Lorimer (Site Lead)
Wesley Van Voorhis (Co-Principal Investigator)
Lynn Barrett (Lab Manager)
Gabriele Varani (Co-Principal Investigator)
Washington State University
Garry Buchko (Co-Principal Investigator)
David Veesler (Co-Principal Investigator)
David Baker (Co-Principal Investigator
Lance Stewart (Co-Investigator)
Neil King (Co-Investigator)
This team is tasked with ensuring efficient and effective transfer of data and information between all SSGCID members and are primarily involved in design and maintenance of the SSGCID SharePoint site, SSGCID website and the SSGCID Central Target Tracking Database (CTTDB). Team members are also involved with maintaining site-specific databases.
The Target Selection team will carry out two or three rounds of target selection each year, usually resulting in batches of 100-300 protein targets and process targets requested by the community.
The cloning & expression teams at each site will design PCR primers to amplify or synthesize target genes, clone them into the appropriate vectors, and test them for soluble expression in bacterial, wheat-germ, cell-free, baculovirus and mammalian expression systems.
CIDResearch - Cell-free expression systems and protein-protein complexes
Beryllium - Bacterial, baculovirus and mammalian expression systems
UW-PPG - Bacterial expression systems
The protein production teams at each site will grow large-scale preps of solubly-expressed proteins and purify milligram quantities for crystallization screening and NMR-based HSQC analysis.
Crystallization testing of purified proteins from all Protein Production Teams will be carried out at Beryllium using a high-throughput robotic system.
This team involves personnel at three sites and includes three different processes (X-ray crystallography, nuclear magnetic resonance and cryo-electron microscopy).
Beryllium - X-ray crystallography
UW NMR Group - NMR
Washington State University - NMR
UW CryoEM Group - CryoEM
This team involves personnel at the Institute of Protein Design.