As part of the NIAID contract, SSGCID and CSGID offer a protein gene-to-structure service to the infectious disease scientific community. All materials and information generated from these services will become publicly available. Structures will be deposited with the Protein Data Bank and materials are available on our Available Materials page. Additionally, we submit data about our targets to database resources such as the PSI and TargetTrack. Applicants will be expected to execute Blanket Confidentiality Agreements (CDA) and Material Transfer Agreements (MTA) with SSGCID and CSGID, similar to the documents used in PSI-2 (http://www.nigms.nih.gov/Initiatives/PSI/).
Individual or collaborative groups of investigators interested in proposing a target for structure determination at the SSGCID or CSGID are requested to submit a Target Selection Proposal to the appropriate center (see below).
Please note that our pipeline is most successful with soluble proteins or domains (no trans-membrane regions) shorter than 700 residues and containing at most 10 cysteines.
Applicants wishing to request targets from the following organisms should use the CSGID site.
Bacilli (Bacillus anthracis, Enterococcus, Listeria, Staphylococcus, Streptococcus)
Gamma-proteobacteria (Coxiella, Escherichia, Francisella, Salmonella, Shigella, Vibrio, Yersinia)
Epsilon-proteobacteria (Campylobacter, Helicobacter)
dsDNA viruses (BK virus, JC virus, Orthopoxvirus, Rhadinovirus, Roseolovirus)
ssDNA virus (Erythrovirus)
ssRNA positive-strand viruses (Alphavirus, Caliciviridae, Coronavirus incl. Betacoronavirus, Enterovirus, Flavivirus, Hepacivirus, Hepatovirus, Hepevirus)
Applicants wishing to request targets from the following organisms should use the SSGCID site
Alpha-proteobacteria (Anaplasma, Bartonella, Brucella, Ehrlichia, Rickettsia)
Spirochaetes (Borrelia, Leptospira)
Eukaryotic pathogens (Absidia, Acanthamoeba, Aspergillus, Babesia, Balamuthia, Coccidioides, Crotococcus, Cryptosporidium, Cunninghamella, Cyclospora, Encephalitozoon, Entamoeba, Giardia, Mucor, Naegleria, Rhizopus, Toxoplasma, Giardia)
ssRNA negative-strand viruses (Arenavirus, Ebola-like viruses, Hantavirus, Henipavirus, Influenzavirus A, Influenzavirus B, Influenzavirus C, Lyssavirus A, Marburgvirus, Measles virus, Nairovirus, Orthobunyavirus, Phlebovirus, Rubulavirus)
Submission may be done by filling out the online web form or by e-mailing a document describing the properties and importance of the protein, availability of genomic or cDNA from the target organism, and how determination of its structure will benefit the scientific community. Applications will receive a proposal number and an acknowledgement of receipt will be e-mailed to the applicant. Proposed targets will be reviewed on a monthly basis by the SSGCID or CSGID Target Selection teams, using similar criteria to those applied to targets selected internally and approved by the NIAID. Approved applications will be given the highest priority for entry into the HTP structure determination pipeline, and the applicants will receive periodic e-mails updating their progress.
To view a list of all community request targets in the SSGCID pipeline, click here.
Nucleoside diphosphate kinase from Borrelia burgdorferi prepared in a transition-state complex with ADP and vanadat… https://t.co/ESh4gPtH7f
3 months ago