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RNA Methyltransferase

  • 2’-O-methyl RNA methyltransferase:
    The 2’-O-methylation of ribosomal RNA is one of the most common ways bacteria can obtain antibiotic resistance. The structure of the 2’-O-methyl RNA methyltransferase from B. pseudomallei (BupsA.00072.a) contains a 31 (trefoil) protein knot. According to the protein knot server (http://knots.mit.edu/), which confirmed that this structure has a knot, there are only 40 similar structures in the PDB, including several SpoU-like RNA methyltransferases. The fold of these RNA methyltransferases is quite different from the classical methyltransferase fold, although related to the other SpoU-like RNA methyltransferases. The ribbon diagram in the figure shows it to be a dimer and the thread of the knot can be seen as the orange-red section passing through the yellow-green section in the bottom dimer. Most SpoU-like RNA methyltransferases also contain an RNA binding domain (RBD), but this RNA methyltransferase does not contain this domain, suggesting it may bind an accessory protein, or perhaps target a different substrate than other enzymes of this family. For more information, please see the Protein Data Bank entry 3e5y.

Cartoon representation of 3e5y structure

Summary of Progress

Cumulative status by number of Unique Targets

*to date

Featured Structures

Overlay of 3 Bartonella BID domains

Bartonella BID

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