Seattle Structural Genomics Center for Infectious Disease


SSGCID SARS-CoV-2 updates

The outbreak of novel coronavirus SARS-CoV-2 (2019-nCoV), which causes COVID-19, was first reported from Wuhan, China on 31 December 2019.  The World Health Organization has declared a global emergency and the SSGCID has rapidly prioritized structural genomic efforts to focus on the elucidation of important protein structures of SARS-CoV-2.

Available data

The sequences of the 10 open reading frames in the SSGCID pipeline were obtained from the GenBank complete genome of isolate Wuhan-Hu-1. Our experimental structure determination efforts are focused on selected targets and progress will be updated here. As with all our targets, expression clones, purified proteins and structure coordinates will be made available to the scientific community.

In particular, the Spike protein of betacoronaviruses is involved in virus entry into the host cell. The Spike model was requested by Richard Scheuerman from the Virus Pathogen Database (ViPR) to map human betacoronaviruses B cell/antibody epitopes that are conserved in the Wuhan-Hu-1 isolate; details will be posted on the ViPR 2019-nCov newspage.

model structure of Spike protein

Model of  the SARS-CoV-2 Spike protein (Baker Lab) with conserved epitopes highlighed in green; superimposed glycans from the SARS CryoEM structure (Veesler Lab) in blue.